TRITIUM, RADIOACTIVE:  Nuclear Power Plant Emissions
CASRN: 10028-17-8

Effluent Concentrations :
The World Health Organization has estimated the total amount of tritium released from atmospheric testing of nuclear weapons through 1980 was approximately 1.2X10+20 to 2.4X10+20 Bq(1). The total environmental discharge of tritium in the effluent of pressurized water reactor (PWR), boiling water reactor (BWR), heavy water reactor (HWR) and gas cooled reactor (GCR) nuclear power plants operating worldwide in 1980 was estimated as 4X10+15 Bq(1). The total tritium activity discharged into the environment by nuclear fuel reprocessing plants operating in Windscale, UK, La Hague, France and Marcoule, France were 2X10+15 Bq in 1978(1). Annual tritium airborne release rates from the Savannah River Plant in South Carolina have ranged from 1.4X10+16 Bq to 9.9X10+16 Bq from 1974 to 1977 with an average of 4.1X10+16 Bq(1). An accidental pulse release of approximately 2X10+13 Bq of tritium from an industrial plant in Northern Switzerland moved through the sewers, to a sewage treatment plant, and was ultimately released to the River Glatt(2).
[(1) WHO; Environmental Health Criteria 25. Geneva, Switzerland, WHO (1983) (2) Hoehn E; pp. 3-10 in Groundwater Quality. IAHS publ. No. 250, Herbert M, Kovar K, eds, IAHS Press: Wallingford, UK (1998) ]**PEER REVIEWED**


Environmental Fate/Exposure Summary :
There are 6 isotopes of hydrogen /tritium/. Hydrogen-1 and hydrogen-2 are naturally occurring and stable. Hydrogen-3 is naturally occurring and radioactive. Hydrogen isotopes with mass numbers 4-6 are artificially produced and are radioactive. Tritium is the a naturally occuring radioactive isotope of hydrogen and is produced as a result of the interaction of cosmic radiation with gasses present in the upper atmosphere. The natural steady-state global inventory of tritium is about 7.3 kilograms. It is naturally present as a very small percentage of ordinary hydrogen in water (HTO), both liquid and vapor and tritium gas (HT). Tritium has also been released to the environment from past atmospheric testing of nuclear weapons and from discharges from nuclear power plants. HTO is generally indistinguishable from normal water and is transported throughout the environment in the same manner as water. Rain and snowfall remove tritium from the atmosphere and volatilization of HTO from water and soil surfaces transports tritium back to the troposphere. In soil, tritium is transported by runoff, erosion and leaching. Tritium is eventually transformed to He-3 by the natural process of radioactive decay with a half-life of about 12.26 years. Occupational exposure may occur through inhalation and dermal contact with this compound at workplaces where tritium is produced or used. Monitoring data indicate that the general population may be exposed to tritium via ingestion of water and food, and dermal contact with tritium in rainfall and atmospheric samples. (SRC)
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General Manufacturing Information :
The United States has not produced tritium since 1988, when the Department of Energy's (DOE's) production facility site in South Carolina closed. Immediate tritium needs are being met by recycling tritium from dismantled U.S. nuclear weapons. According to DOE, resumption of tritium production is essential for maintaining the U.S. nuclear weapons stockpile. ... On December 22, 1998, the DOE Secretary announced that he had chosen the light water reactor technology as the primary means for tritium production; the accelerator design will be retained as a backup. He selected the Tennessee Valley Authority's (TVA's) Watts Bar Nuclear Plant and Sequoyah Nuclear Plant, Units 1 and 2, in Tennessee as the preferred facilities for producing future supplies of tritium. ... The tritium will still be shipped to (in the form of tritium producing burnable absorber rods) and extracted at the Savannah River site.
[U.S. Nuclear Regulatory Commission; Backgrounder on Tritium Production (June 2005) Available at http://www.nrc.gov/reading-rm/doc-collections/fact-sheets/tritium-bg.html as of September 27, 2006 ]**PEER REVIEWED**


Non-Human Toxicity Excerpts :
/LABORATORY ANIMALS: Neurotoxicity/ To provide experimental data for evaluating tritium risk of nuclear-energy plant and instituting safety protection standard of nuclear effects of low-dose tritiated water(HTO) on the developing central nerve system, ... Pregnant adult C57BL/6J strain mice and Wistar rats were irradiated with beta-rays from HTO by a single intraperitoneal injection on the 12.5th and 13th day of gestation. The activity of HTO was (x10+4) Bq/g b.w.) 24.09, 48.18 and 144.54, respectively. Neurobehavior and learning and memory ability were tested. Ca2+ currents of hippocampal neurons were examined by patch-clamp. Apoptosis of brain cells was detected morphologically and biochemically by DNA electrophoresis. SDS-PAGE and Western blotting were used to detect P53 protein in the cultures. ... After irradiation with beta-rays from HTO in utero, the offspring showed hyperactivity in their young age period and then showed depression-hypoactivity in adulthood. The exposed animals had difficulties in both learning and memory retention and the developing neurons of the central nerve system showed lower Ca2+ currents, higher number of apoptosis, a typical ladder pattern DNA and increasing P53 protein. /The authors concluded that/ Lower Ca2+ currents, higher number of apoptosis of brain cells and abnormal expression of P53 protein were the substance basis of the damage of central nerve system caused by tritium.
[Zhou X et al; ZHonghua Yi Xue Za Zhi 79 (7): 493-6 (1999) ]**PEER REVIEWED**


Ecotoxicity Excerpts :
/PLANTS/ Maize and barley plants were grown from seed for a period of 30 days in an enclosure in which the soil water and atmospheric vapor contained equilibrium concentrations of tritiated water. At the end of the experiment the plant water of the maize and the barley contained 95 and 84%, respectively, of the environmental concentration of tritium. The tritium-to-hydrogen ratio in plant dry matter was 60% for maize and 45% for barley of the environmental tritium-to-hydrogen ratio. The results show a significant isotope effect which reduces the tritium content of food grown in a continuously contaminated environment.
[Garland JA, Ameen M; Health Physics 36 (January): 35-8 (1979) ]**PEER REVIEWED**


Other Chemical/Physical Properties :
DECAY PATHWAY: Hydrogen-3, half-life 12.32 years, decays via beta(-) emission (18.590 keV) to helium-3, half-life stable
[IAEA; NuDat 2.1. Brookhaven National Laboratory. National Nuclear Data Center. Inter Atomic Energy Agency, Nuclear Data Sect. Vienna, Austria. Available at http://www-nds.iaea.org/nudat2/index.jsp as of Jul 28, 2006. ]**PEER REVIEWED**


Natural Pollution Sources :
A significant amount of tritium from the sun's surface is believed to be brought to the earth by solar wind and flare emissions. This tritium is rapidly incorporated into water molecules and mixed into the water of the atmosphere, hydrosphere, and biosphere(1).
[(1) Kohman TP; Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984. v19: 667 (1982) ]**PEER REVIEWED**


Clinical Laboratory Methods :
Methods of Analysis. Tritium is collected primarily as/ water vapor/ HTO along with water (H2O) by distillation and then determined from its beta emission in a liquid scintillation system. No gamma rays are emitted. The distillation process is usually performed from a basic solution of potassium permangenate to oxidize radionuclides and organic compounds, preventing them from distilling over and subsequently interfering with counting. Charcoal can also be added to the distillation mixture as an additional measure to remove organic material. Volatile iodine radionuclides can be precipitated as silver iodide before distillation. Another distillation technique involves the use of cyclohexane to form an azeotropic (low boiling point) mixture. This technique is sometimes used in analysis of biota samples. Tritium may be analyzed, indirectly, by mass spectrometry of its progeny, 3He.
[Multi-Agency Radiological Laboratory Analytical Protocols Manual Volume II: Chapters 10-17 and Appendix F. (July 2004) p 14-180 NUREG-1576, EPA 402-B-04-001B, NTIS PB2004-105421. Available at http://www.nrc.gov/reading-rm/doc-collections/nuregs/staff/sr1576/sr1576v2.pdf as of October 12, 2006 ]**PEER REVIEWED**


Major Uses :
Bombarding particle in cyclotrons, activator in self-luminous phosphors, in cold cathode tubes, tracer in biochemical research and various special problems in chemical analysis, luminous instrument dials, thermonuclear power research.
[Lewis, R.J. Sr.; Hawley's Condensed Chemical Dictionary 14th Edition. John Wiley & Sons, Inc. New York, NY 2001., p. 1140]**PEER REVIEWED**


Artificial Pollution Sources :
Tritium's use as a radioactive agent in making luminous paints(1-3), bombarding particle in cyclotrons, activator in self-luminous phosphors, in cold cathode tubes, tracer in biochemical research and various special problems in chemical analysis, luminous instrument dials, and thermonuclear power research(2) may result in its release to the environment through various waste streams(SRC).
[(1) Lide DR; CRC Handbook of Chemistry and Physics 86th ed 2005-2006. CRC Press, Taylor & Francis, Boca Raton, FL p. 4-18 (2005) (2) Lewis RJ Sr; Hawley's Condensed Chemical Dictionary 14th ed. John Wiley & Sons, Inc. New York, NY p. 1140 (2001) (3) Argonne National Laboratory/EVS; Human Health Fact Sheet, August 2005. Tritium (Hydrogen-3). Available at: http://www.ead.anl.gov/pub/doc/tritium.pdf as of Jul 25, 2006 ]**PEER REVIEWED**


General Manufacturing Information :
The principal USA site of production is the Savannah River Plant near Aiken, SC. /Former/
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present., p. V8: 23 (1993)]**PEER REVIEWED**


Plant Concentrations :
Tritium was detected in floral samples of three different species of plants in the vicinity of radioactive waste lagoons located in Los Alamos, NM at levels of 3.3 to 78.0 pCi/mL(1).
[(1) Haarmann TK; Arch Environ Contam Toxicol 35: 287-294 (1998) ]**PEER REVIEWED**


Artificial Pollution Sources :
... Present in effluents from nuclear reactors and weapons. Currently, the tritium present in the environment and the relative contribution of the sources have been estimated to be about 0.5 to 1 megacurie from nuclear reactors ... and about 1X10+3 megacuries from nuclear explosions.
[Doull, J., C.D.Klassen, and M.D. Amdur (eds.). Casarett and Doull's Toxicology. 3rd ed., New York: Macmillan Co., Inc., 1986., p. 697]**PEER REVIEWED**


Medical Surveillance :
Tritium is a radionuclide uniformly absorbed by the body. The purpose of cytomorphological and cytochemical leukocytes examination in tritium-affected workers was early detection of initial health disorder caused by tritium contamination. Cytoplasm and nuclear morphological changes were analyzed by colored blood smear microscopy. The enzyme activity in granulocytes was examined by cytochemical methods. Morphological changes were found in 47.17% of the subjects (N=53) and in 2% (N=52) of the controls. Chromosomal aberrations in lymphocytes were found in 49.05% of the exposed workers and in 2% of the controls. The granulocytes enzyme activities were significantly diminished in the exposed workers (p < or =0.001). The average level of beta radiation found in the urine of those affected by tritium was 3.46 kBq/L, while it was significantly lower in the others: 0.35 kBq/L. The enzyme activity decreases after 5 yr of tritium exposure. Nuclear and cytoplasm changes as well as lowered enzyme activity in granulocytes were found in tritium-contaminated workers. Interdependence of cytomorphological and cytochemical changes was established. There was no correlation between cytochemical changes and granulocytes count.
[Milacic S; Health Phys 86 (5): 457-9 (2004) ]**PEER REVIEWED**


Human Toxicity Excerpts :
/BIOMONITORING/ /GENOTOXICITY/ Tritium is a radionuclide uniformly absorbed by the body. The purpose of cytomorphological and cytochemical leukocytes examination in tritium-affected workers was early detection of initial health disorder caused by tritium contamination. Cytoplasm and nuclear morphological changes were analyzed by colored blood smear microscopy. The enzyme activity in granulocytes was examined by cytochemical methods. Morphological changes were found in 47.17% of the subjects (N=53) and in 2% (N=52) of the controls. Chromosomal aberrations in lymphocytes were found in 49.05% of the exposed workers and in 2% of the controls. The granulocytes enzyme activities were significantly diminished in the exposed workers (p < or =0.001). The average level of beta radiation found in the urine of those affected by tritium was 3.46 kBq/L, while it was significantly lower in the others: 0.35 kBq/L. The enzyme activity decreases after 5 yr of tritium exposure. Nuclear and cytoplasm changes as well as lowered enzyme activity in granulocytes were found in tritium-contaminated workers. Interdependence of cytomorphological and cytochemical changes was established. There was no correlation between cytochemical changes and granulocytes count.
[Milacic S; Hlth Phys 86 (5): 457-9 (2004) ]**PEER REVIEWED**


RCRA Requirements :
Under the implementing regulations of the Resource Conservation and Recovery Act (RCRA) [specifically at 40 CFR 261.4(a)(4)], source, special nuclear and by-product material as defined by the Atomic Energy Act of 1954 (AEA) is excluded from the definition of solid waste (and thus, from the RCRA hazardous waste management requirements). ... Accelerator-produced tritium, on the other hand, does not qualify for this exclusion (since the tritium is produced by a linear accelerator, and does not involve the production or utilization of special nuclear materials or the extraction or concentration of source material). ... Pursuant to the RCRA regulations, it is the responsibility of the generator of a waste to determine if that waste is subject to the hazardous waste requirements [40 CFR 262.11].
[USDOE; DOE Handbook Tritium Handling and Safe Storage DOE-HDBK-11290YrXXXX-YR p. 20 (December 1991). Available from http://www.eh.doe.gov/techstds/tsdrafts/doe-hdbk-1129-yr.pdf as of July 29, 2006 ]**PEER REVIEWED**


Methods of Manufacturing :
Numerous reactions are available for the artificial production of tritium and it is now made on a large scale by neutron irradiation of enriched lithium-6 in a nuclear reactor. The lithium is in the form of an alloy with magnesium or aluminum which retains much of the tritium until it is released by treatment with acid. Alternatively the tritium can be produced by neutron irradiation of enriched LiF at 450 degrees in a vacuum and then recovered from the gaseous products by diffusion through a palladium barrier.
[Greenwood, N.N.; Earnshaw, A. (1998). Chemistry of the Elements (2nd Edition). p. 41. Elsevier. Online version available at: http://www.knovel.com/knovel2/Toc.jsp?BookID=402&VerticalID=0 ]**PEER REVIEWED**


Methods of Manufacturing :
A small quantity of tritium is produced through neutron capture by deuterium in the heavy water used as moderator in /nuclear/ reactors.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present., p. V8: 23 (1993)]**PEER REVIEWED**


Methods of Manufacturing :
Tritium is produced as a minor product of nuclear fission. The yield of tritium is 1-2 atoms in 10,000 fissions of natural uranium, enriched uranium, or a mixture of transuranium nuclides.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present., p. V8: 24 (1993)]**PEER REVIEWED**


Other Chemical/Physical Properties :
Magnetic dipole moment: +2.97896 nuclear magnetons
[Lide, D.R. CRC Handbook of Chemistry and Physics 86TH Edition 2005-2006. CRC Press, Taylor & Francis, Boca Raton, FL 2005, p. 11-51]**PEER REVIEWED**


Other Chemical/Physical Properties :
Reacts with deuterium or protons (at sufficiently high temp) to undergo nuclear fusion.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present., p. V8: 19 (1993)]**PEER REVIEWED**


Preventive Measures :
Surveys of hood exhausts to unrestricted areas may be necessary to demonstrate compliance with NRC (Nuclear Regulatory Commission) or state regulations.
[Fuscaldo, A., B. J. Erlick, and B. Hindman. (eds.). Laboratory Safety-Theory and Practice. New York: Academic Press, 1980., p. 102]**PEER REVIEWED**


Non-Human Toxicity Excerpts :
/LABORATORY ANIMALS: Subchronic or Prechronic Exposure/ Tritium (3-H) may enter the environment from human activities, particularly at production, processing, or waste storage sites such as the Department of Energy's Savannah River Site, a former nuclear production facility in South Carolina. Understanding the dynamics and potential adverse effects of tritium in exposed organisms is critical to evaluating risks of tritium releases at such sites. Previous studies estimated the biological half-life of tritium in mice to be approximately 1.13 days; however, these laboratory studies were not conducted under environmentally realistic conditions. In this study, designed to be more representative of environmental exposure, mice were allowed to drink water containing tritium (activity about 300 Bq/mL) for a period of 2 wk. The induction of oxidative stress from tritium exposure was evaluated by comparing the activities of antioxidant enzymes (catalase, glutathione peroxidase, and superoxide dismutase) in exposed and control mice. From this experiment, the biological half-life of tritium was determined to be 2.26+/-0.04 days, almost double previous estimates. While positive controls (X-ray irradiated mice) showed responses in antioxidant enzyme activity, there was no indication of oxidative stress induction in mice exposed to tritium at this concentration.
[Kelsey-Wall A et al; J Toxicol Environ Health A 69 (3-4): 201-13 (2006) ]**PEER REVIEWED**


Natural Pollution Sources :
Tritium occurs naturally to the extent of about 1 atom per 10+18 hydrogen atoms(1) as a result of nuclear reactions induced by cosmic rays in the upper atmosphere(1,2).
[(1) Katz JK; in Kirk-Othmer Encyclopedia of Chemical Technology. (2005). NY, NY: John Wiley & Sons; Deuterium and Tritium. Online Posting Date: Feb 13, 2004. (2) Argonne National Laboratory/EVS; Human Health Fact Sheet, August 2005. Tritium (Hydrogen-3). Available at: http://www.ead.anl.gov/pub/doc/tritium.pdf as of Jul 25, 2006 ]**PEER REVIEWED**


Natural Pollution Sources :
The principal source of natural tritium is the nuclear reactions induced by cosmic radiation in the upper atmosphere, where fast neutrons, protons, and deuterons collide with components of the stratosphere to produce tritium. Tritium has also been observed in meteorites(1).
[(1) Katz JK; in Kirk-Othmer Encyclopedia of Chemical Technology. (2005). NY, NY: John Wiley & Sons; Deuterium and Tritium. Online Posting Date: Feb 13, 2004. ]**PEER REVIEWED**


Environmental Water Concentrations :
In the prenuclear age, tritium content of rainwater ranged from < 1 to > 100 TUs (Tritium Units), depending mainly on the time between evaporation from seawater and precipitation of the water mass. Continental river water and biosphere hydrogen typically contained several TUs. The well mixed surface layer of the oceans generally had a few tenths of a TU, and the deep sea waters had undetectable levels. At the height of atmopsheric testing of nuclear weapons in 1963, the tritium content of rainwater attained a maximum of about 1X10+4 TUs in the northern hemisphere and about a tenth as high in the southern hemisphere. The content of surface seawater increased to about 5-20 TU. /TU= Tritium Unit= 1 (3)H atom/1X10+18 H atoms, corresponding to a specific radioactivity in water of about 199 mBq/kg (7.1 dpm/kg)/(1).
[(1) Kohman TP; Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984. V19: 667 (1982) ]**PEER REVIEWED**


Food Survey Values :
Tritium was detected in 4% of 200 portions of foods (raw vegetables, fruits, fish, and milk) collected near 33 nuclear reactors from October 1986 to September 1992(1). The maximum concentration observed in these positive detections was 70 Bq/kg, and most of the positive detections occurred in fish and vegetables in the vicinity of 4 sites(1).
[(1) Cunningham WC et al; J AOAC Int 77: 1422-1427 (1994) ]**PEER REVIEWED**


Federal Drinking Water Standards :
Pursuant to the Atomic Energy Act, discharges and releases of tritium to waters from DOE facilities are regulated by DOE and those from licensed nuclear facilities are regulated by NRC or NRC Agreement States. Under the Safe Drinking Water Act, the Environmental Protection Agency (EPA) has established the standard for tritium in water from drinking water systems. EPA and States also use this standard for protection of ground water that may be used as drinking water. DOE requirements prohibit DOE operations from releasing effluents that would cause a drinking water system to exceed this standard.
[DOE; Stakeholder Sensitivity to Tritium Releases.DOE EH/-0699, June 2006. Available at www.eh.doe.gov/paa/advisory/SAd_2006-04.pdf as of July 2006. ]**PEER REVIEWED**


Analytic Laboratory Methods :
Fourier transform NMR can be used to detect (3)H (nuclear spin one-half), which has an efficiency of detection /of approximately/ 20% greater than that of (1)H. This technique is useful for ascertaining the position and distribution of tritium in the labeled compound.
[Kirk-Othmer Encyclopedia of Chemical Technology. 4th ed. Volumes 1: New York, NY. John Wiley and Sons, 1991-Present., p. V20 865 (1996)]**PEER REVIEWED**

MORE ABOUT HEALTH EFFECTS

TRITIUM, RADIOACTIVE
CASRN: 10028-17-8
This record contains information for tritium, an isotope of hydrogen. For general information on radiation, such as transportation, sampling, analytical methods, regulations, and spill clean-up, refer to the IONIZING RADIATION record.
For other data, click on the Table of Contents


Human Toxicity Excerpts:
/HUMAN EXPOSURE STUDIES/ The body does not readily absorb H2, HT, HD, D2, DT, or T2 from inhaled gases or through the skin. If inhaled in elemental form, almost all tritium in the gas is exhaled. Only a very small fraction is retained in the lungs. Tritium in the form of water (HTO, DTO, and T2O) is adsorbed through the skin and in the lungs from inhaled gases. Tritium in water form is readily retained in the body and remains with a biological half-life of approximately 10 days. Due to the body's ready adsorption of tritium in the form of tritiated water, exposure to tritiated water in air is on the order of 15,000 to 25,000 times more hazardous than exposure to gaseous tritium (HT, DT, and T2).
[USDOE; DOE Handbook Tritium Handling and Safe Storage DOE-HDBK-11290YrXXXX-YR p. 5 (December 1991). Available from http://www.eh.doe.gov/techstds/tsdrafts/doe-hdbk-1129-yr.pdf as of July 29, 2006 ]**PEER REVIEWED**

/CASE REPORTS/ A woman was accidentally exposed to tritiated gas that escaped from glass capillary tubes. She incorporated about 35 GBq (1 Ci) of tritiated water. After several hours of delay, treatment was initiated to reduce the exposure by enhanced fluid intakes and forced diuresis. She was followed for chromosomal aberrations for 11 years. The authors monitored the number of dicentric chromosomes found in lymphocytes, and found that they have fallen over the years with a half-time of 3.3 years. No other significant clinical abnormalities were noted.
[CaEPA/OEHHA; Public Health Goals for Chemicals in Drinking Water: Tritium p.16 (March 2006). Available from: http://www.oehha.ca.gov/water/phg/pdf/PHGtritium030306.pdf as of July 25, 2006. ]**PEER REVIEWED**

/CASE REPORTS/ Urine samples from eight male radiation workers who had an unplanned acute tritiated water intake were measured for tritium-in-urine up to 300 d post-exposure. During the first month or so post-exposure, these individuals increased their fluid intakes to accelerate the turnover rate of tritium in the body for dose mitigation. Their daily fluid intakes reverted to normal levels in the latter period of the study. A non-linear regressional analysis of the tritium-in-urine data showed that the average biological half-life of tritium in body water, with standard deviation, was 6.3+/-1.0 days (range, 5.0-8.1 d) and 8.4+/-2.0 days (range, 6.2-12.8 d) during the respective periods of increased fluid intake and the later period of normal fluid intake. A longer term component of tritium excretion was also observed with average biological half-life of 74+/-18 days (range, 58-104 d), indicating the incorporation of tritium, and its retention, in the organic fractions of the body. A mathematical model was developed and used to estimate the dose increase from the metabolized organically bound tritium on the basis of the kinetics of tritium-in-urine. The model accounts for a change in the rates of urinary excretion caused by variable fluid intakes. The average dose to the body, for the eight male workers, due to the metabolized organically bound tritium was estimated to be 6.2+/-1.3% (range, 3.5% to 8.9%) of the committed effective dose due to tritium in the body water. This value for the dose increase from organically bound tritium is in the range of the current recommendations of the International Commission on Radiological Protection, i.e., organically bound tritium incorporated into the body contributes about 10% of the dose to the body water following tritiated water intakes.
[Trivedi A et al; Hlth Phys 73 (4): 579-86 (1997) ]**PEER REVIEWED**

/BIOMONITORING/ For bioassay purposes, urine is normally used for determining tritium concentrations in body water. Workers who may be or who have been exposed to tritium are normally required to submit urine samples for bioassay periodically. The sampling period may be daily, biweekly, or longer, depending on the potential for significant exposure. Special urine samples are normally required after an incident or a work assignment with a high potential for exposure. After a possible exposure, the worker should empty the bladder 1 to 2 hours later. A sample taken after the bladder is emptied should be reasonably representative of the body water concentration. A sample collected before equilibrium is established will not be representative because of dilution in the bladder, or because of initial high concentration in the blood. However, any early sample may still be useful as a sign of the potential seriousness of the exposure.
[USDOE; Primer on Tritium Safe Handling Practices DOE-HDBK-1079-94 p.18 (December 1994). Available from: http://www.eh.doe.gov/techstds/standard/hdbk1079/hdbk1079.pdf as of July 26, 2006. ]**PEER REVIEWED**

/BIOMONITORING/ /GENOTOXICITY/ Tritium is a radionuclide uniformly absorbed by the body. The purpose of cytomorphological and cytochemical leukocytes examination in tritium-affected workers was early detection of initial health disorder caused by tritium contamination. Cytoplasm and nuclear morphological changes were analyzed by colored blood smear microscopy. The enzyme activity in granulocytes was examined by cytochemical methods. Morphological changes were found in 47.17% of the subjects (N=53) and in 2% (N=52) of the controls. Chromosomal aberrations in lymphocytes were found in 49.05% of the exposed workers and in 2% of the controls. The granulocytes enzyme activities were significantly diminished in the exposed workers (p < or =0.001). The average level of beta radiation found in the urine of those affected by tritium was 3.46 kBq/L, while it was significantly lower in the others: 0.35 kBq/L. The enzyme activity decreases after 5 yr of tritium exposure. Nuclear and cytoplasm changes as well as lowered enzyme activity in granulocytes were found in tritium-contaminated workers. Interdependence of cytomorphological and cytochemical changes was established. There was no correlation between cytochemical changes and granulocytes count.
[Milacic S; Hlth Phys 86 (5): 457-9 (2004) ]**PEER REVIEWED**

/OTHER TOXICITY INFORMATION/ Tritium ... does not constitute an external radiation hazard. However, tritium presents a serious hazard through ingestion and subsequent exposure of vital body tissue to internal radiation.
[Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984., p. 7(79) 561]**PEER REVIEWED**

/OTHER TOXICITY INFORMATION/ The median lethal dose (LD50) of tritium assimilated by the body is estimated to be 370 GBq (10 Ci). Higher doses can be tolerated with forced fluid intake to reduce the biological half life.
[Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984., p. 7(79) 561]**PEER REVIEWED**

/OTHER TOXICITY INFORMATION/ Risk coefficients for inhalation of radionuclides are expressed as the probability of radiogenic cancer mortality or mobidity per unit intake, where the intake is averaged over all ages and both genders. ...Mortality and morbidity risk coefficients for inhalation /of Hydrogen (H-3, particulate), fast absorption type and gastrointestinal uptake of 1.0 are/ 3.61X10-13 Bq-1 and 5.28X10-13 Bq-1, /respectively/. /For medium absorption type and GI uptake of 0.1 the risk coefficients are/ 4.58X10-12 Bq-1 and 5.38X10-12 Bq-1. /For slow absorption and GI uptake of 0.01 the risk coefficients are/ 2.12X10-11 Bq-1 and 2.30X10-11 Bq-1. Mortality and morbidity risk coefficients for inhalation /of Hydrogen (H-3, water vapor) with GI uptake of 1.0 are/ 1.04X10-12 Bq-1 and 1.52X10-12 Bq-1 /respectively/. /For Hydrogen (H-3, elemental gas) with GI uptake of 1.0 are/ 1.04X10-16 Bq-1 and 1.52X10-16 Bq-1. /from table/
[USEPA/Air and Radiation; Fed Guidance Rpt No. 13: Cancer Risk Coefficients for Environmental Expsoure to Radionuclides EPA 402-R-99-001 p.21-22 (September 1999) ]**PEER REVIEWED**

/OTHER TOXICITY INFORMATION/ Risk coefficients for ingestion of radionuclides in tap water or food are expressed as the probability of radiogenic cancer mortality or morbidity per unit intake, where the intake is averaged over all ages and both genders. ...Mortality and morbidity risk coefficients for ingestion of water and food /for Hydrogen (H-3, tritiated water) with a gastrointestinal uptake of 1.0:/ Tap water intakes- 9.44X10-13 Bq-1 and 1.37X10-12 Bq-1 /respectively/; Dietary intakes- 1.20X10-12 Bq-1 and 1.76X10-12 Bq-1. Mortality and morbidiey risk coefficients for ingestion of water and food /for Hydrogen (H-3, organically bound) with a GI uptake of 1.0:/ Tap water intakes- 2.09X10-12 Bq-1 and 3.03X10-12 Bq-1, /respectively/; Dietary intakes- 2.66X10-12 Bq-1 and 3.89X10-12 Bq-1. /from table/
[USEPA/Air and Radiation; Fed Guidance Rpt No. 13: Cancer Risk Coefficients for Environmental Expsoure to Radionuclides EPA 402-R-99-001 p.83-84 (September 1999) ]**PEER REVIEWED**


Human Toxicity Values:
The median lethal dose (LD50) of tritium assimilated by the body is estimated to be 370 GBq (10 Ci). Higher doses can be tolerated with forced fluid intake to reduce the biological half life.
[Kirk-Othmer Encyclopedia of Chemical Technology. 3rd ed., Volumes 1-26. New York, NY: John Wiley and Sons, 1978-1984., p. 7(79) 561]**PEER REVIEWED**


Probable Routes of Human Exposure:
NIOSH (NOES Survey 1981-1983) has statistically estimated that 5,947 workers (1,110 of these are female) are potentially exposed to tritium in the US(1). Occupational exposure may occur through inhalation and dermal contact with this compound at workplaces where tritium is produced or used(SRC). Monitoring data indicate that the general population may be exposed to tritium via ingestion of water and food, and dermal contact with tritium in rainfall and atmospheric samples(SRC). Human exposure to tritium most often occurs in the form of tritiated water, THO(2).
[(1) NIOSH; NOES. National Occupational Exposure Survey conducted from 1981-1983. Estimated numbers of employees potentially exposed to specific agents by 2-digit standard industrial classification (SIC). Available at http://www.cdc.gov/noes/ as of Jul 25, 2006. (2) WHO; Environmental Health Criteria 25. Geneva, Switzerland, WHO (1983) ]**PEER REVIEWED**


Probable Routes of Human Exposure:
NIOSH (NOES Survey 1981-1983) has statistically estimated that 5,947 workers (1,110 of these are female) are potentially exposed to tritium in the US(1). Occupational exposure may occur through inhalation and dermal contact with this compound at workplaces where tritium is produced or used(SRC). Monitoring data indicate that the general population may be exposed to tritium via ingestion of water and food, and dermal contact with tritium in rainfall and atmospheric samples(SRC). Human exposure to tritium most often occurs in the form of tritiated water, THO(2).
[(1) NIOSH; NOES. National Occupational Exposure Survey conducted from 1981-1983. Estimated numbers of employees potentially exposed to specific agents by 2-digit standard industrial classification (SIC). Available at http://www.cdc.gov/noes/ as of Jul 25, 2006. (2) WHO; Environmental Health Criteria 25. Geneva, Switzerland, WHO (1983) ]**PEER REVIEWED**

All of the above is directly from http://toxnet.nlm.nih.gov/

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